To valorize Pleurotus eryngii mycelium protein (PeMP) as a sustainable protein source, this study investigated the efficacy of ultrasound-assisted extraction (0-450 W) in simultaneously enhancing the extraction yield and modulating the physicochemical properties of PeMP. Results showed that ultrasound treatment at 150 W achieved a maximal extraction yield of 76.23%, significantly outperforming the alkali-soluble acid precipitation method. Structural characterization, including SDS-PAGE and LC-MS/MS, revealed that ultrasonic cavitation induced controlled protein unfolding without disrupting the primary peptide chain. This process reduced the content of rigid α-helices and significantly increased surface hydrophobicity. These conformational changes markedly improved the interfacial behavior of PeMP: appropriately ultrasound-modified (150 and 300 W) fractions exhibited superior solubility, emulsifying activity, and foaming stability compared to commercial soy protein isolate (SPI), while maintaining comparable water- and oil-holding capacities. Although the essential amino acid ratio was slightly lower than that of SPI, it fully met the FAO/WHO requirements for adult nutrition. Furthermore, the structural loosening exposed enzyme cleavage sites, leading to a significant enhancement in vitro digestibility. Overall, this study demonstrates that ultrasound-assisted extraction acts as a robust dual-function strategy for producing high-quality mycelium protein with tailored functionalities, offering a viable plant-based alternative for the food industry.
Kai Zhou, Qingyue Wang, Qiaozhi Li et al.· International Journal of Bio...· 0 citations
The association between immunocyte phenotypes and dilated cardiomyopathy (DCM) has been explored, however the exact pathogenesis of the relationship between immune cells and DCM is unclear. This bidirectional two-sample Mendelian randomization (MR) research aims to further validate the causal link between 731 immunocyte phenotypes and DCM. Summary statistics from a genome-wide association study data of individuals with European ancestry were utilized, including 1444 DCM cases and 353,937 controls, as well as 3757 European adults for the 731 immunocyte phenotypes. Causal effects were estimated using inverse variance weighted, MR-Egger regression, weight median estimator, weighted mode, and simple mode. Sensitivity analysis was conducted to confirm data robustness and feasibility. Based on the inverse variance weighted findings, 14 immunocyte phenotypes were risk factors for DCM (P < .05, odds ratio [OR] > 1), while 15 immunocyte phenotypes exhibited a protective effect on DCM (P < .05, OR < 1). The results of reverse MR analysis suggested evidence that DCM occurrence might elevate the levels of 17 immunocyte phenotypes (P < .05, OR > 1) and decrease the levels of 9 immunocyte phenotypes (P < .05, OR < 1). Our research indicated that CD28 on secreting regulatory T cell could mitigate the occurrence of DCM, and reciprocally, the progression of DCM could reduce the level of CD28 on secreting regulatory T cell. This study confirmed the bidirectional genetic predictive relationship between immunocyte phenotypes and DCM, underscoring the complex interplay between DCM and the immune system.
Wenshuai Feng, Xindi Chang, Qiaozhi Li et al.· Medicine· 0 citations
