Qin-Yu Ge

Phosphorylation-asymmetric recombinase polymerase amplification enables target-directed circularization for ultrasensitive multiplex electrochemical detection of respiratory pathogens

Amplification bias in sequencing-based spatial transcriptomics: sources, mechanisms, impacts, and mitigation strategies

Abstract Spatial transcriptomics (ST) has emerged as a powerful approach for profiling gene expression in spatial tissue context; yet, its quantitative accuracy remains substantially compromised by amplification bias introduced during the complex library preparation process. These biases arise at multiple stages and ac...