Heterologous Expression of a Thermostable Laccase from Chrysoporthe cubensis in Komagataella phaffii Improves Thermal Stability and Dye Decolorization.
The phytopathogenic fungus Chrysoporthe cubensis was cultivated on agro-industrial residues to evaluate laccase production, with coffee husks inducing the highest activity. The enzyme, identified as laccase MCO12, was purified and biochemically characterized, showing acidic activity (pH 2.2-5.0), optimum at 55 °C, and...