SPECTROPHOTOMETRIC ASSESSMENT OF VITAMIN B2 (RIBOFLAVIN) BY DIAZOTIZATION AND COUPLING REACTION WITH 2-AMINO-4-(3-PYRIMIDYL) PYRIMIDINE REAGENT
Objective: The diazotization and coupling method were employed as a simple and sensitive spectrophotometric analysis technique for the determination of riboflavin. Method: The method is based on the reaction between diazotization of the reagent using sodium nitrite in acidic medium and the maximum absorption wavelength...