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OpenKinetics Predictor (predictor.openkinetics.org), an open-source platform integrating thirteen kinetic parameter prediction methods accessible via one interface, is presented, finding inter-predictor agreement varies with metabolic context and data availability.
Predicting enzyme kinetics directly from sequence remains a central challenge in computational biology, particularly in resolving the effects of mutations at catalytically essential residues. Existing models frequently overlook the functional consequences of such perturbations, defaulting to wild-type predictions eve...
This study investigates the effects of pBHR1’s native mobilization protein, MobV, on the retention of pBBR1 origin plasmids in R. palustris, and provides design principles for constructing stable, high-performing vectors in non-model gram-negative hosts.
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