Boosting Cytochrome P450 Electro-Catalysis: Substrate-Dependent Kinetics, Electro-Induced Activation, and NADH/NAD+ Ratio Control.
Efficient electron supply and cofactor utilization remain key bottlenecks in cytochrome P450-catalyzed oxidations. Here, we report an electro-assisted system that overcomes these limitations, using the P450 Ema1-catalyzed oxidation of avermectin B1a as a model reaction. Under a constant current of 1 mA, > 96% conversio...