Open access
Sep 2026
De novo engineered guide RNA-directed transposition with TnpB-family proteins
Summary Programmable DNA integration using CRISPR-associated transposase elements (CASTs) offer powerful capabilities for genome engineering. The large single effector Cas12k CAST examples evolved from a minimal TnpB nuclease protein. Here, we engineer a de novo RNA-guided transposition systems in bacteria, where the s...
Richard D. Schargel, Laura Chacon Machado, S. Kumaran et al.
· Molecules and Cells · 0 citations