Author

Rick Russell

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Open access Sep 2025

Structural basis for target discrimination and activation by Cas13d

The mechanisms of nuclease activation are explored by solving seven ternary cryo-electron mi-croscopy structures of wild-type Cas13d in complex with matched and mismatched targets and an active site loop in the HEPN domains that regulates substrate accessibility is identified.

Chia-Wei Chou, Selma Sinan, Hung-Che Kuo et al. · bioRxiv · 0 citations

Open access Jul 2026

Rho (𝛒) Analysis to Dissect RNA Folding and Assembly Pathways

The results establish ρ analysis as a general framework to probe RNA conformational pathways and function and uncover multiple folding pathways and modulation in pathway flux that are readily understood from the energetic properties of the constituent RNA motifs.

Brant Gracia, Sarah E Nielson, Daniel Herschlag et al. · bioRxiv · 0 citations