Human Induced Pluripotent Stem Cell-Derived Cardioids as a Model to Assess Oligonucleotide Delivery.
A detailed, step-by-step protocol is presented for generating self-organizing, 3D cardioids from human induced pluripotent stem cells and applying them as a platform to evaluate the uptake of fluorescently labeled oligonucleotides, supporting the development and optimization of delivery strategies for cardiac applicati...