Robert T. Kennedy

Machine vision-enabled OD600 measurement and mass spectrometric determination of metabolites of microbial cell-containing microfluidic droplets in real-time

Droplet microfluidics has become a versatile tool for high-throughput phenotypic analysis of cells, employed in enzyme engineering, drug screening, and antibiotic assays. An important metric in such bioanalyses is the optical density (OD600) of the culture, but current methods for measuring OD600 within cultured drople...