Structural insights into sequential phthalic acid ester degradation: Co-crystal structures of a dialkyl/monoalkyl phthalate hydrolase with intermediate and product.
Phthalic acid esters (PAEs) are persistent pollutants with environmental and health concerns. Dialkyl/monoalkyl PAEs hydrolases can fully degrade PAEs, offering sustainable bioremediation potential, yet their bifunctional mechanism remains unclear. This study resolved co-crystal structures of EstJ6 bound to catalytic i...