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Author

Ru-Yue Wei

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Sep 2026

A steric hindrance-regulated autocatalytic CRISPR/Cas12a system for direct and amplification-free microRNA detection.

We constructed a CRISPR/Cas12a autocatalytic system based on a miRNA-triggered overhang blocker (OB) cleavage-recombination framework, in which Cas12a sequentially removed steric hindrance to initiate cyclic autocatalysis, enabling exponential signal amplification for visual miRNA detection without target pre-amplifica...

Wei-Su Kong, Jia-Xuan Li, Ru-Yue Wei et al. · 0 citations

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