ent-Kaurene, ent-kauran-16-ol, and shunted diterpenoids from an engineered strain of Aspergillus oryzae
Aspergillus oryzae was engineered to produce high levels of geranylgeranyldiphosphate (GGPP) and ent-kaurene, via ent-copalyl diphosphate, as precursors for future metabolic engineering projects via the expression of ggs2 and cps/ks genes from Gibberella fujikuroi under the control of strong constitutive promoters. GGP...