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S. Lees-Miller

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Open access Sep 2026

Tandem-Affinity Crosslinking Mass Spectrometry Supports a Model of a DNA-PKcs-PP6 Regulatory Complex.

Protein complexes can be modeled with data arising from crosslinking mass spectrometry, but it is difficult to collect such data from cellular sources, particularly for proteins that are part of extensive interaction networks. Here, we present a tandem-affinity purification crosslinking mass spectrometry (xTAP‑MS) meth...

Danny Laurent, Morgan F. Khan, Morgan Hepburn et al. · 0 citations
Open access Sep 2026

DNA-PK driven nucleosome unwrapping enables NHEJ in chromatin

DNA double-strand breaks, one of the most cytotoxic forms of DNA damage, are primarily repaired by non-homologous end joining (NHEJ) in human cells. NHEJ is initiated by the Ku70/80 heterodimer (Ku) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs), followed by factors that bridge and ligate DNA ends. Ku...

Wei-Fen Lu, Alex Vogt, S. Lees-Miller et al. · 0 citations

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