Sequential enzymatic pretreatment and Saccharomyces boulardii fermentation of cactus cladode biomass for bioactive food ingredients and cell culture serum alternatives.
It is demonstrated that DL + SC pretreatment of CCB combined with SB fermentation can produce bioactive food ingredients and candidate protein-rich growth supplements for cell culture applications and bioactivity and serum replacement evidence remains partial, in vitro and restricted to a single cell line.