Precision phenomics is introduced as a unifying framework that links molecular, spatial, functional, and clinical characteristics of tumors to support personalized cancer management and has the potential to transform lung cancer research and improve patient outcomes.
The modulatory role of Sirtuins in FOXP3 expression and Treg differentiation is reviewed, providing insight into their contribution to tumor immunosuppression and integrating network-based analysis identified miR-34a as a central regulator and validated the miRNA-Transcription Factor-Sirtuin axis as a viable framework for cancer therapeutic targeting.
Aishwarya Saha, Sriparna De, Sreya Chattopadhyay et al.· Molecular Biology Reports· 0 citations
Strategic bioconversion of lignocellulosic agro-wastes like sugarcane bagasse (SCB) into high-value Riboflavin is a rarely reported phenomenon. The present study explored a Riboflavin-producing bacterial strain,
Microbacterium proteolyticum
BWBTDIPO1 (GenBank Acc no.: PQ517523), isolated from the dumping area of Kolkata, West Bengal for its caliber to utilize SCB as a source of carbon and energy for the sustainable bio-production of the valuable nutraceutical under submerged fermentation (SmF) conditions. A strong association of bacterial growth (specific growth rate of μ = 0.325 h
-1
) with production of Riboflavin (yield of 397 ± 15.8 mgL
-
1, which amounts to 19.85 ± 2.0 mg per gram of SCB after 78 h) was recorded. Yeast extract peptone mineral salt media (YPMSM) was found to be the most suitable medium for the production. UV-Vis spectrophotometry and thin-layer chromatography (TLC) with an R
f
value of 0.83 confirmed that the metabolite was Riboflavin. The biochemical assays revealed that the strain utilized SCB components, including lignin, cellulose, and hemicellulose. Further confirmation of biomass deconstruction was assessed via FTIR, XRD, and FESEM analysis, where reports ensured successful deconstruction of SCB by the bacterial isolate. To complement the experimental findings, KEGG-based
in silico
pathway analysis was performed to explore potential metabolic routes associated with lignocellulosic biomass utilization and carbon metabolism. The predicted pathways provide a hypothetical framework for understanding biomass deconstruction and require experimental validation. This is the first report of Riboflavin production from waste SCB via bacterial treatment
(M. proteolyticum)
, indicating the novel nature of the strain and the methodology employed. Valorizable Riboflavin obtained from waste SCB can be a green alternative to chemical Riboflavin synthesis as well as bulk SCB waste management, promoting the concept of microbe-mediated waste-to-wealth conversion, thus contributing to circular bioeconomy.
Indrani Paul, S. Kali, Sonia Saha et al.· Frontiers in Chemical Engine...· 0 citations