Open access
Aug 2026
Suboptimal PAM Enables One-Pot RPA–CRISPR/Cas12a Detection of Clostridium perfringens in Food Samples
The strict requirement for a canonical TTTV protospacer adjacent motif (PAM) restricts target-site selection in clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 12a (CRISPR/Cas12a)-based diagnostics and limits the flexibility of assay design. Whether suboptimal PAMs can be effectively...
Bo Li, Xin Li, Kai Li et al.
· Current Issues in Molecular... · 0 citations