Shintaro Iwasaki

RNA-dependent RNA amplification as a strategy to perform tRNA sequencing on low-input samples

Transfer RNAs (tRNAs) are key molecules that deliver amino acids to the translating ribosome according to their cognate codon encoded in messenger RNA (mRNA). Due to the modified nature of tRNA nucleotides, accurate tRNA quantification can be tedious, especially when dealing with small sample inputs. Here, we took adva...

Halting translation by the letter with sequence-selective small molecules.

Neuronal stop-codon readthrough is associated with ribosome pausing and alters protein localization in Drosophila

An in vivo atlas of neuron-specific ribosome profiling datasets in Drosophila suggests that neuronal RT is associated with structured post-stop RNA regions that reshape termination dynamics and can produce IDR-rich C-terminal protein extensions with distinct subcellular localization.