Single-molecule insights into SAM-I riboswitch ligand binding using scaled magnetic force spectroscopy with comparison to conventional biophysical methods
ABSTRACT Biophysical methods are widely used for drug discovery, with surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) being amongst the most used techniques. However, these types of bulk or ensemble methods do not provide single-molecule resolution or structural and mechanistic insights, so t...