NAD⁺-driven modular multi-enzyme cascade system enables the in vitro synthesis of 3-hydroxypropionic acid from glucose.
3-Hydroxypropionic acid (3-HP) is an important bio-based platform chemical with broad industrial potential. Most reported in vitro biosynthetic systems rely on costly NADPH, which limits scalability. Here, a modular in vitro multi-enzyme cascade pathway was designed using D-glucose as the sole carbon source, driven exc...