Author

Shuli Liang

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Review Open access Sep 2026

Systematic optimization of Komagataella phaffii (Pichia pastoris) for high-efficiency recombinant protein production: Advancements in strain engineering, fermentation optimization, and AI-driven technologies

Komagataella phaffii (formerly Pichia pastoris) has emerged as an indispensable eukaryotic expression host for the production of industrial enzymes, food proteins, and therapeutic proteins owing to its Generally Recognized as Safe (GRAS) status, efficient protein secretion capacity, eukaryotic post-translational modifi...

Yue Huang, Jia-Liang Wang, Ao-Xue Wang et al. · Engineering Microbiology · 0 citations

Open access Aug 2026

Metabolic Engineering of Komagataella Phaffii for de Novo Synthesis of Retinol From Methanol

Retinol, a derivative of vitamin A with potent antioxidant and therapeutic properties, is in high market demand. In response to the low productivity of conventional methods, metabolic engineering has been explored for microbial retinol production. However, systematic engineering strategies for high‐level retinol synthe...

Yan-Xuan Wu, Chen-Guang Liu, Shu-Li Liang et al. · Biotechnology Journal · 0 citations

Aug 2026

Reprogramming Komagataella phaffii Cell Factory for High-Yield Production of 3S,3′S-Astaxanthin via Multiplex Metabolic Engineering and Its Application

The synthesis of 3S,3′S-astaxanthin was successfully and effectively applied in shrimp farming for color enhancement and antioxidant effects and will pave the way for astaxanthin industrial production.

Xinying Zhang, Ji-Yuan Hu, Yan-Xuan Wu et al. · ACS Synthetic Biology · 0 citations