Malonyl-CoA is a major extender unit in polyketide biosynthesis; however, in Escherichia coli, it is predominantly consumed by fatty acid biosynthesis, limiting its availability for heterologous polyketide production. Here, we engineered a fatty acid biosynthesis-β-oxidation carbon-recycling pathway (FABOX pathway) tha...
Understanding of exometabolite-mediated interactions in S. pombe is advanced by identifying GSH as an exometabolite that influences cellular processes and potentially shapes microbial communities by analyzing gene deletion mutants whose growth is supported by extracellular GSH.
A systematic functional analysis of VibH, a stand-alone C domain VibH from the vibriobactin biosynthetic pathway, reveals distance-dependent steric tolerance and mechanistic insights into C-domain specificity and establishes a framework for rational NRPS reprogramming.
F. Ishikawa, Hiroyuki Izuno, Shinya Nakamura et al.· ACS Chemical Biology· 0 citations
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