Si-Jia Tang

2-photon fluorescence microscopy of brain cells beyond 2 mm.

3-photon microscopy (3 PM) has been the dominant technology for in vivo imaging of deep brain cells down to the hippocampus. However, tissue attenuation and rapid 3 PM signal decay limit the imaging depth to 1.5 mm. Here, we demonstrate non-invasive 2-photon fluorescence (2PF) imaging to a depth of 2 mm in the mouse br...