Sian A. Martin

CRISPR-Mediated Gene Editing: Generating Gene Knockouts or Precision Base Changes in F0 Xenopus.

The complete process of producing a gene knockout or precision base changes in F0 Xenopus is described: target selection, design and synthesis of sgRNA, base editor selection and synthesis of base editor mRNA, microinjection into fertilized Xenopus eggs, and genotyping to assess whether gene editing has successfully oc...