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The modularity of the system enables incorporation of additional niche components, such as monocytes, allowing investigation of the monocyte–glia–tumor axis and providing a powerful platform to dissect mechanisms regulating the GB microenvironment.
The results demonstrate that EPM1 COs display increased electrophysiological activity and disrupted excitatory/inhibitory (E/I) balance, and identify the CSTB-SHH-EV axis as a potential therapeutic target for mitigating the E/I imbalance associated with EPM1.
A reproducible human three-dimensional brain tissue model with neurons, astrocytes and microglia, replicating in-vivo-like maturation and enabling the study of Alzheimer’s disease-relevant perturbations and drug responses is developed.
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