ESIPT-induced selective tryptophan detection: a multifunctional probe for biomacromolecule binding with theoretical and biocompatibility studies.
A coumarin-based fluorescent ligand, 4-hydroxy-2-oxo-2H-chromene-3-carbaldehyde (HCC), was synthesized and evaluated for the selective detection of tryptophan (Trp) among the nine essential amino acids. The probe exhibited remarkable selectivity toward Trp through an excited-state intramolecular proton transfer (ESIPT)...