Malonyl-CoA is a major extender unit in polyketide biosynthesis; however, in Escherichia coli, it is predominantly consumed by fatty acid biosynthesis, limiting its availability for heterologous polyketide production. Here, we engineered a fatty acid biosynthesis-β-oxidation carbon-recycling pathway (FABOX pathway) tha...
The meta-selective C–H functionalization of aromatic substrates lacking meta-directing electron-withdrawing groups remains challenging. To engineer the regioselective enzymatic conversion of biphenyl (BP) to 3,3′-dihydroxybiphenyl (33DHBP), an industrial precursor to high-performance polymers, substrate positioning w...
C. Vavricka, Takeshi Matsui, Satoshi Yuzawa et al.· ACS Catalysis· 0 citations
This study demonstrates PHBH biosynthesis in R. capsulatus and indicates that limited 3HHx-CoA supply rather than polymerization capacity is the primary bottleneck, providing a foundation for further pathway and host optimization toward flexible PHBH production from diverse substrates.
Kako Miura, Takayuki Shimizu, T. Hasunuma et al.· Journal of Bioscience and Bi...· 0 citations
This chapter focuses on research progress in metabolic engineering of BT production, which mainly includes design of BT biosynthetic pathways for expression in bacterial and yeast cell factories, current metabolic engineering and process optimization strategies to optimize BT production, and utilizing lignocellulosic b...
Weibin Wang, Mengrui Tao, Kai Li et al.· Advances in Biochemical Engi...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
