Author

T. Tiso

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Open access Sep 2026

Enhancing acetate-based biosurfactant production by Pseudomonas putida KT2440 through fermentation optimization

Background There is an increasing demand for sustainable chemicals, although “green premium” can be rarily achieved. In this context, biosurfactants emerge as promising substitutes for petroleum- or palm-oil-based surfactants due to their potentially lower environmental impact. 3-(3-hydroxyalkanoyloxy)alkanoic acids (H...

Carina V. Michel, Christopher Weike, Laura N. Breuer et al. · Microbial Cell Factories · 0 citations

Open access Aug 2026

Improving acetate metabolism of P. putida KT2440 by evolutionary and rational engineering

Acetate is a promising carbon source for microbial biotechnology as it can be produced sustainably from lignocellulosic biomass or C1 gases. Since acetate is directly activated to acetyl-CoA, it is especially suitable for producing acetyl-CoA-derived products, showcased here with the production of 3-(3-hydroxyalkanoylo...

M. Filbig, Luisa Wachtendonk, Luca A. I. Hampe et al. · bioRxiv · 0 citations

Open access Aug 2026

Adaptive laboratory evolution rewires Pseudomonas putida for resource-efficient acetate assimilation

Acetate is an attractive renewable two-carbon substrate for microbial biotechnology, but its toxicity limits growth and carbon-use efficiency at process-relevant concentrations. Here, we used adaptive laboratory evolution to improve acetate tolerance in a genome-reduced strain of Pseudomonas putida and combined whole-g...

N. Gurdo, A. Srinivasan, Tommaso Tagliani et al. · bioRxiv · 0 citations