Semi-rational Engineering of Xylulokinase from Lactococcus lactis N8 for Enhanced Catalytic Efficiency via Synergistic Hotspot Mutations
Efficient xylose bioconversion is limited by the catalytic performance of xylulokinase (XylB), which phosphorylates d-xylulose to xylulose-5-phosphate. This study aimed to improve XylB from Lactococcus lactis N8 using a multi-dimensional semi-rational design strategy integrating sequence conservation, structure-based...