A Single-Tube Dual-Gene RAA-PfAgo Assay for Simultaneous Detection and Identification of PEDV Field and Vaccine Strains.
Porcine epidemic diarrhea virus (PEDV) poses a significant threat to the swine industry, and distinguishing circulating field strains from vaccine strains under field conditions remains challenging. In this study, we established a one-tube visual detection platform based on recombinase‑aided amplification (RAA) and PfA...