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Review Sep 2026

Decoding microbial fructosyltransferases: Structural insights, molecular engineering, and biotechnological applications.

Fructosyltransferase (FTF), classified within glycoside hydrolase families GH32 and GH68 of the GH-J clan, plays an indispensable catalytic role in the biosynthesis of fructooligosaccharides (FOS) and fructan polymers (such as inulin and levan). These enzymes specifically catalyze fructosyl transfer reactions, construc...

Meng-Li Li, Xue-Yan Han, Ming Miao et al. · 0 citations
Sep 2026

Computationally assisted semirational engineering of Nicotiana attenuata α-glucan phosphorylase enhances cellobiose-to-amylose cascade synthesis.

Converting non-food cellulosic sugars into programmable amylose offers a route to higher-value carbohydrate materials. α-Glucan phosphorylase (αGP) controls glucose-1-phosphate (G-1-P)-dependent chain elongation in cellobiose-to-amylose cascades. Here, Nicotiana attenuata αGP (NicαGP) was engineered by integrating Alph...

Wei Gao, Meng-Li Li, Ming Miao et al. · 0 citations

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