Author

Thea C T Irvine

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2026

A Golden Gate-Compatible CRISPR-Associated Transposon Tool for Multiplexed Bacterial Genome Editing.

An improved tool called pSPIN-GG and supporting protocols for simplified CAST-based genome engineering are presented and refinements support accelerated library construction, reduce assembly and screening burden, and expand the accessibility of CAST systems for multiplexed bacterial genome engineering.

Thea C T Irvine, Andrew M Bailey, Thomas E. Gorochowski · Methods in molecular biology · 0 citations