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This chapter presents a detailed protocol for performing in vitro LLPS assays, particularly using polyethylene glycol (PEG) and sodium salt as key modulators of phase behavior.
This chapter explores the properties of tau, including its intrinsically disordered nature, isoforms, and post-translational modifications (PTMs), that predispose it to LLPS, and examines how truncation and PTM cross-talk alter the phase behavior and aggregation propensity of tau.
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