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Author

Tina Vodopivec Seravalli

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Open access Oct 2026

Development of Immobilized Restriction Enzyme Reactors on a Monolith Backbone for Streamlined Plasmid DNA Linearization.

High-quality, properly linearized plasmid DNA (pDNA) is a decisive upstream determinant of in vitro transcription (IVT) performance, impacting mRNA yield, integrity, and batch-to-batch consistency. We developed immobilized restriction enzyme reactors (IMERs) on monolithic supports and benchmarked EcoRI, NdeI, and HindI...

Maša Nabergoj, Špela Peršič Debenjak, Polona Skrt et al. · 0 citations

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