Ting-Ting Xiang

A Two-Step Strategy Based on Fpg Recognition and RCA-CRISPR/Cas12a for Ultrasensitive Detection of Gene Mutation

Accurate detection of gene mutations is crucial for acute myeloid leukaemia (AML) diagnosis and treatment. Although CRISPR/Cas systems enable sensitive mutation detection, their adaptability is constrained by dependence on restriction endonucleases. Herein, we propose a two-step strategy, termed Fpg-RCA-CRISPR/Cas12a...