Milk-Clotting Enzyme from Bacillus velezensis DS-1 (Longnan Douchi): Purification, Structure Prediction, and Cheese Making.
This study isolated a high milk-clotting enzyme-producing strain DS-1 from Longnan Douchi, identified as Bacillus velezensis based on 16S rDNA. The enzyme was purified by ammonium sulfate precipitation and DEAE-Sephadex A-25 chromatography, yielding 56.1 kDa, 6.57-fold purification, and 68.57% recovery. It showed optim...