Author

Tomoko Miyata

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Open access Aug 2026

Cryo-EM structure of CYP2C9 reveals a dimer-of-trimers assembly

Human cytochrome P450 2C9 (CYP2C9) is a hepatic microsomal enzyme involved in the oxidative metabolism of clinically important drugs, but the structural organization of its oligomeric assemblies outside crystallographic packing environments remains poorly understood. Here, we report the cryo-EM structure of human CYP2C...

Hiroki Tanino, Hirofumi Tsujino, Teruhiro Nakao et al. · bioRxiv · 0 citations

Open access Sep 2026

Haloplasma MreB reconstitution in a minimal synthetic bacterium reveals orthogonal force directions underlying distinct Mollicute motility systems

MreB, a bacterial actin homolog, is widely conserved in bacteria where it functions as a scaffold for a peptidoglycan synthesis. Intriguingly, members of the cell wall-lacking Mollicutes retain multiple mreB genes despite the absence of peptidoglycan. Previous work demonstrated that two MreB isoforms from Spiroplasma c...

Mone Mimura, Haruka Yuasa, Hirofumi Wada et al. · bioRxiv · 0 citations

Open access Aug 2026

ADP-Bound States of Cytoplasmic Dynein: Cryo-electron Microscopy Reveals a Two-Step Post-Power-Stroke Transition and Roles of Regulatory ATPase Sites.

These findings provide a mechanistic model in which coordinated conformational dynamics across multiple ATPase sites govern dynein's chemomechanical cycle and suggest a dynamic equilibrium between two ADP-bound states.

Hiroshi Imai, R. Kanazawa, Toshihisa Maeshima et al. · Journal of Molecular Biology · 0 citations