Development of a TaqMan-Based Multiplex Real-Time RTPCR Assay for Detection of Mosquito-Borne Viral Diseases
Background: Mosquito-borne viral diseases, including Dengue (DENV), Zika (ZIKV), Chikungunya (CHIKV), Yellow fever (YFV), and West Nile (WNV), represent a significant global health burden. Existing singleplex molecular assays are often time-consuming and resource-intensive. We aimed to develop and analytically validate...