Oral carcinoma is a major global health concern and a leading cause of carcinoma-related mortality. This study evaluated a methanolic polyherbal extract for its phytochemical composition and biological efficacy, along with mechanistic insights via in silico network pharmacology. GC-MS and UPLC profiling confirmed diverse bioactive compounds, particularly polyphenols, with UV peaks at 272 nm. The extract showed strong antioxidant activity (IC₅₀ = 108 ± 0.17 µg/mL) and significant antimicrobial effects (inhibition zones: 6.00–21.33 mm). Cytotoxicity assays revealed anticancer activity against OECM-1 oral carcinoma cells (IC₅₀ = 18.86 µg/mL). The polyphenolic fraction suppressed pro-inflammatory cytokines (IL-6, IL-10, TNF-α, IL-1β, TGF-β) with IC₅₀ values of 3.02–6.15 µg/mL. Network pharmacology and molecular docking indicated multi-target interactions of key phytochemicals in pathways relevant to oral carcinoma progression. These findings suggest the polyherbal extract may be a complementary candidate in oral carcinoma management via antioxidant, anti-inflammatory, and antiproliferative effects.
E. Mary, L. Inbathamizh, V. Pandey et al.· Scientific Reports· 2 citations
Food security and environmental sustainability have emerged as major global challenges, driving the search for alternative protein production systems that can meet growing demand while reducing the environmental burden of conventional livestock farming. Among emerging alternatives, cultivated meat has gained considerable attention due to its potential to produce animal-derived protein without the need for large-scale animal rearing and slaughter. Although cultivated meat remains absent from most commercial markets owing to technical, economic, and regulatory challenges, significant milestones have been achieved, including regulatory approval in Singapore in 2020, completion of the U.S. Food and Drug Administration pre-market safety consultation in 2022, and authorization for commercial sale by the U.S. Department of Agriculture in 2023. Substantial progress has been made in cell sourcing, cell-line engineering, serum-free culture media development, scaffold fabrication, and bioprocess optimization. However, large-scale production of cultivated meat with desirable texture, flavor, nutritional quality, and economic viability remains challenging. Key bottlenecks include the development of cost-effective animal-component-free media, scalable bioreactor systems, edible scaffold materials, and efficient downstream processing strategies. This review provides an updated assessment of recent advances in cultivated meat technology, encompassing cell-line development, scaffold engineering, bioprocessing, post-processing approaches, sustainability considerations, and regulatory frameworks. Furthermore, current technological limitations, commercialization challenges, and future research priorities are discussed to evaluate the potential role of cultivated meat within sustainable and resilient future food systems.
Zaryab Shafi, P. Nath, V. Pandey et al.· Biotechnology and Bioenginee...· 0 citations
