Review
Open access
Oct 2026
Normalization Strategies for miRNA RT-qPCR in Blood-Derived Samples in Obesity Research: Current Practices and Methodological Considerations
Reverse transcription quantitative PCR (RT-qPCR) is widely used to quantify circulating microRNAs, but reliable interpretation depends on appropriate data normalization. In obesity research, selecting reference RNAs for measurements in whole blood, blood cells, plasma, and serum is challenging because of biological het...
A. Pustovalova, A. F. Nikolaeva, A. Kalinkin et al.
· International Journal of Mol... · 0 citations