INCLUSION BODIES IN RECOMBINANT PROTEIN PRODUCTION IN ESCHERICHIA COLI: FORMATION, RECOVERY, AND IMPLICATIONS FOR DOWNSTREAM PROCESSING
Recombinant protein production in Escherichia coli is a well-established biotechnology platform, but intense heterologous expression frequently results in inclusion bodies. These aggregates are not necessarily deposits of completely misfolded protein: they may retain native-like structural elements and, in selected sys...