Open access
Sep 2026
Culture media alter retinal organoid physiology promoting AAV transduction and retinal ganglion cell survival.
Human iPSC-derived retinal organoids offer a human-relevant platform for inherited retinal disease (IRD) gene therapy, yet robust AAV transduction in vitro remains challenging. Here we show that culture in BrainPhys™ (BP) medium markedly enhances AAV-mediated gene delivery. Brief BP exposure during the transduction win...
Michelle O'Hara-Wright, Benjamin Y. Lim, Melissa M. Mangala et al.
· Gene Therapy · 0 citations