W. N. Zagotta

Measuring protein conformational changes with genetically encoded bipyridylalanine and transition-metal ion FRET

Transition-metal ion Förster resonance energy transfer (tmFRET) measures short-range distances and conformational changes in proteins, but its distance sensitivity depends on the donor– acceptor pair. Here, we establish the metal-chelating noncanonical amino acid bipyridylalanine (bpyA) as a flexible, genetically encod...