Aug 2026
A Novel, Simple, and Highly Efficient Dual-sgRNA CRISPR/Cas9-Based Editing System in the Filamentous Fungus Fusarium oxysporum
A novel highly efficient CRISPR/Cas9-based dual-sgRNA expression editing system for F. oxysporum suitable not only for single-gene editing but also for large-fragment deletion and multiplex gene editing, although the editing efficiency is somewhat lower.
Wangjie Zhu, Jiao Liao, Yuanyuan Liu et al.
· ACS Synthetic Biology · 0 citations