LhSiase1-catalyzed transglycosylation: unlocking the bioactive potential of bird's nest fragments for sialylated oligosaccharides synthesis.
Human milk oligosaccharides (HMOs) comprise a diverse array of structurally complex glycans, among which 3'-sialyllactose (3'-SL) is a predominant bioactive sialylated oligosaccharide. In this study, a novel sialidase LhSiase1 from Lactobacillus helsingborgensis was characterized and applied in a one-pot hydrolysis-tra...