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Author

Weidong Liu

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Open access Oct 2026

Structural and catalytic characterization of the 2,5-dihydroxypyridine ring-cleaving dioxygenase Hpo from Pseudomonas putida S16

ABSTRACT 2,5-Dihydroxypyridine (2,5-DHP) is a potentially carcinogenic intermediate in pyridine catabolism. Hpo, a non-heme Fe(II)-dependent dioxygenase from Pseudomonas putida S16, catalyzes ring cleavage of 2,5-DHP, yet its structure has remained uncharacterized. We determined the crystal structure of Hpo at 2.56 Å r...

Zhen-Yu Cheng, Xu Han, Hong-Li Wei et al. · 0 citations
Open access Jul 2026

Crystal structure of a fungal dihydroxyacetone kinase reveals a non-canonical ATP-binding site.

Dihydroxyacetone kinase (DAK) catalyzes the ATP-dependent phosphorylation of dihydroxyacetone (DHA) and is an important enzyme in artificial starch synthesis. Here, we report the crystal structure of a methylotrophic yeast DAK from Komagataella phaffii (formly Pichia pastoris, PpDAK). ATP was observed at a non-canonica...

Hongli Wei, Yangyang Chen, Fan Zhang et al. · 0 citations

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