d-Allulose is a promising low-calorie rare sweetener; however, its industrial production is limited by the insufficient catalytic activity and thermostability of d-allulose 3-epimerases (DAEases). In this study, a combinatorial engineering strategy integrating lid-domain modulation and computational design was develope...
Qi-Chen Liu, Xin-Rui Tang, Wen-Jing Sun et al.· Journal of Agricultural and...· 0 citations
Simultaneously enhancing the thermostability and catalytic activity of acetylxylan esterases (AxEs) remains a significant challenge due to the inherent stability-activity trade-off. Here, a previously uncharacterized CE7 family acetylxylan esterase (TsAxE) from Thermoclostridium stercorarium was identified and engineer...
Yu-Jiao Tao, Xin-Rui Tang, Mei Zhao et al.· Journal of Agricultural and...· 0 citations
The genetic determinants hypothetically linked to efficient 2KGA synthesis, including glucose metabolism, fatty acid metabolism, and the oxidative phosphorylation system are delineated, which could provide the genomic resource for elucidating high productivity and robustness, and rationally engineering the high-perform...
Lulu Li, Lei Sun, Xin-Yi Zan et al.· Biotechnology for Biofuels a...· 0 citations
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