Spatiotemporal Sequential Fluorescence and Electrochemistry Bimodal Quantitation of Enzyme Activity in a Living Cell.
A spatiotemporal sequential fluorescence-electrochemistry bimodal platform for both spatial mapping and precise quantitation of asparaginyl endopeptidase (AEP) activity in living single cells is developed and offers a powerful tool for studying enzyme heterogeneity, subcellular localization, and dynamic enzymatic activ...