Wendy R. Gordon

Aerolysin enables modular, non-genetic functionalization of living cell surfaces

It is shown that a non-cytotoxic, monomeric aerolysin mutant retains high-affinity and GPI-AP-dependent cell binding when genetically fused to diverse protein cargos and a DNA hairpin sensor is developed that converts cell-surface nuclease activity into a fluorescent signal and distinguishes cells with different levels...