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Author

Wenjing Xie

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Review Aug 2026

CRISPR RNA Engineering Enables Single Nucleotide Polymorphism Discrimination in Nucleic Acid Detection.

This review synthesizes current advances aimed at enhancing the specificity of CRISPR diagnostics with particular emphasis on the pivotal role of CRISPR RNA (crRNA) engineering, and details how structural determinants of crRNA govern CRISPR-mediated target recognition and define the energetic and kinetic thresholds for accurate cleavage.

Tianrui Sun, Aijiao Yuan, Wenjing Xie et al. · 0 citations